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Mucin 5 subtype AC expression and upregulation in the nasal mucosa of allergic rhinitis rats.

Authors: Wang, W  Zheng, M 
Citation: Wang W and Zheng M, Otolaryngol Head Neck Surg. 2012 Dec;147(6):1012-9. doi: 10.1177/0194599812460977. Epub 2012 Sep 12.
Pubmed: (View Article at PubMed) PMID:22972875
DOI: Full-text: DOI:10.1177/0194599812460977

OBJECTIVE: To elucidate the functions of nuclear factor kappa B (NF-kappaB) in mucin hypersecretion in allergic rhinitis (AR), we examined the in vivo effects of an NF-kappaB inhibitor, ammonium pyrrolidine dithiocarbamate (PDTC), on mucin 5 subtype AC (MUC5AC) expression in the nasal mucosa of ovalbumin-sensitized rats. STUDY DESIGN: Randomized animal study. SETTING: Academic medical center. SUBJECTS AND METHODS: Sprague Dawley rats were randomized into a control group (group A), an AR model group (group B), and an AR model treated with an NF-kappaB inhibitor (group C). Rats in groups B and C were sensitized systemically and locally by ovalbumin injection and inhalation, whereas group A was treated with normal saline in place of ovalbumin. Pyrrolidine dithiocarbamate (100 mg/kg/d) was given to group C by intraperitoneal injection for 5 days. NF-kappaBp65, MUC5AC, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6 were detected by immunohistochemistry, Western blotting, enzyme-linked immunosorbent assay, or real-time polymerase chain reaction. RESULTS: NF-kappaB was activated in group B, and significant NF-kappaBp65 protein was expressed in the nucleus of cells from the nasal mucosa, resulting in upregulated transcription from TNF-alpha and IL-6 genes, as well as increased contents of TNF-alpha and IL-6 in the nasal lavage fluids. Pyrrolidine dithiocarbamate inhibited nuclear localization of NF-kappaBp65 and subsequent downregulation of the transcription and secretion of TNF-alpha and IL-6. MUC5AC was upregulated in group B but reduced in a time-dependent manner after inhibition of NF-kappaB activation. CONCLUSION: NF-kappaB activation might induce MUC5AC hypersecretion in AR rats by inflammatory cytokines TNF-alpha and IL-6.


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CRRD Object Information
CRRD ID: 7364746
Created: 2013-09-25
Species: All species
Last Modified: 2013-09-25
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.