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In silico cloning of mouse Muc5b gene and upregulation of its expression in mouse asthma model.

Authors: Chen, Y  Zhao, YH  Wu, R 
Citation: Chen Y, etal., Am J Respir Crit Care Med. 2001 Sep 15;164(6):1059-66.
Pubmed: (View Article at PubMed) PMID:11587997
DOI: Full-text: DOI:10.1164/ajrccm.164.6.2012114

Using a BLAST-searching approach, we identified a mouse expressed sequence tag (EST) clone (AA038672) showing great similarity to the 3' end of the human MUC5B gene. The clone was named "3pmmuc5b-1" after complete nucleotide sequencing (Genbank Accession, AF369933). A subsequent search of the mouse genome database with the 3pmmuc5b-1 sequence identified two overlapping genomic clones (AC020817 and AC020794) that contained the sequence of both 3pmmuc5b-1 and the mouse Muc5ac gene. Like their human homologs, the genomic order of the mouse Muc genes is 5'-Muc5ac-Muc5b-3'. These results suggest that the newly identified EST clone, 3pmmuc5b-1, is part of the 3' portion of the mouse Muc5b gene. In situ hybridization demonstrated that this putative mouse Muc5b message was expressed in a restricted manner in the sublingual gland region of the tongue and the submucosal gland region of the mouse trachea in a normal animal. However, the gene expression was greatly enhanced in airway surface epithelium and the submucosal gland region in ovalbumin-induced asthmatic mice. These results were consistent with previous studies of human airway tissues. We therefore conclude that this newly cloned mouse Muc5b gene could be used as a marker for studying aberrant mucin gene expression in mouse models of various airway diseases.

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CRRD Object Information
CRRD ID: 7364763
Created: 2013-09-26
Species: All species
Last Modified: 2013-09-26
Status: ACTIVE



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