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Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains.

Authors: Otsen, M  Den Bieman, M  Winer, ES  Jacob, HJ  Szpirer, J  Szpirer, C  Bender, K  Van Zutphjen, LF 
Citation: Otsen M, etal., Mamm Genome 1995 Sep;6(9):595-601.
Pubmed: (View Article at PubMed) PMID:8535065

Genetic monitoring is an essential component of colony management and for the rat has been accomplished primarily by using immunological and biochemical markers. Here, we report that simple sequence length polymorphisms (SSLPs) are a faster and more economical way of monitoring inbred strains of rats. We characterized 61 inbred strains of rats, using primer pairs for 37 SSLPs. Each of these loci appeared to be highly polymorphic, with the number of alleles per locus ranging between 3 and 14 and, as a result, all the 61 inbred strains tested in this study could be provided with a unique strain profile. These strain profiles are also used for estimating the degree of similarity between strains. This information may provide the rationale in selecting strains for genetic crosses or for other specific purposes.


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Additional Information

CRRD Object Information
CRRD ID: 737874
Created: 2004-03-24
Species: All species
Last Modified: 2004-03-24
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.