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Annexin 1 mimetic peptide protects against renal ischemia/reperfusion injury in rats.

Authors: Facio FN, JR  Sena, AA  Araujo, LP  Mendes, GE  Castro, I  Luz, MA  Yu, L  Oliani, SM  Burdmann, EA 
Citation: Facio FN Jr, etal., J Mol Med (Berl). 2011 Jan;89(1):51-63. doi: 10.1007/s00109-010-0684-4. Epub 2010 Oct 16.
Pubmed: (View Article at PubMed) PMID:20953576
DOI: Full-text: DOI:10.1007/s00109-010-0684-4

Inflammation is currently recognized as a key mechanism in the pathogenesis of renal ischemia-reperfusion (I/R) injury. The importance of infiltrating neutrophil, lymphocytes, and macrophage in this kind of injury has been assessed with conflicting results. Annexin 1 is a protein with potent neutrophil anti-migratory activity. In order to evaluate the effects of annexin A1 on renal I/R injury, uninephrectomized rats received annexin A1 mimetic peptide Ac2-26 (100 mug) or vehicle before 30 min of renal artery clamping and were compared to sham surgery animals. Annexin A1 mimetic peptide granted a remarkable protection against I/R injury, preventing glomerular filtration rate and urinary osmolality decreases and acute tubular necrosis development. Annexin A1 infusion aborted neutrophil extravasation and attenuated macrophage infiltration but did not prevent tissue lymphocyte traffic. I/R increased annexin A1 expression (assessed by transmission electron microscopy) in renal epithelial cells, which was attenuated by exogenous annexin A1 infusion. Additionally, annexin A1 reduced I/R injury in isolated proximal tubules suspension. Annexin A1 protein afforded striking functional and structural protection against renal I/R. These results point to an important role of annexin A1 in the epithelial cells defense against I/R injury and indicate that neutrophils are key mediators for the development of tissue injury after renal I/R. If these results were confirmed in clinical studies, annexin A1 might emerge as an important tool to protect against I/R injury in renal transplantation and in vascular surgery.

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CRRD Object Information
CRRD ID: 7421585
Created: 2013-11-21
Species: All species
Last Modified: 2013-11-21
Status: ACTIVE



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