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The assay of interleukin 4 in the serum of normal subjects and atopic patients using a novel immunoassay.

Authors: Jones, KP  Morris, RH  Rolf, S  Whatley, J  Davies, BH 
Citation: Jones KP, etal., Cytokine. 1997 Jul;9(7):529-34.
Pubmed: (View Article at PubMed) PMID:9237816
DOI: Full-text: DOI:10.1006/cyto.1996.0197

Interleukin 4 (IL-4) was measured in the serum of normals and mild atopic patients using a high sensitivity ELISA system involving an amplification stage. The method was found to have a limit of detection of 0.02 pg/ml after a square root transformation of the concentration data and to show good reproducibility with coefficients of variation between 11 and 14%. Recoveries of standard IL-4 from serum was greater than 90%. Markedly elevated levels of IL-4 were seen in the atopic groups compared with the normals. No association was found between total white cell counts, lymphocyte or eosinophil counts and serum IL-4 levels. This study demonstrates that serum IL-4 levels can distinguish between atopic subjects and normals, even in mild or symptomless disease. This may prove valuable in the management of atopic disorders.

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CRRD Object Information
CRRD ID: 7829817
Created: 2014-01-28
Species: All species
Last Modified: 2014-01-28
Status: ACTIVE



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