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Methylation of RAR-beta2, RASSF1A, and CDKN2A genes induced by nickel subsulfide and nickel-carcinogenesis in rats.

Authors: Zhang, J  Zhang, J  Li, M  Wu, Y  Fan, Y  Zhou, Y  Tan, L  Shao, Z  Shi, H 
Citation: Zhang J, etal., Biomed Environ Sci. 2011 Apr;24(2):163-71. doi: 10.3967/0895-3988.2011.02.011.
Pubmed: (View Article at PubMed) PMID:21565688
DOI: Full-text: DOI:10.3967/0895-3988.2011.02.011

OBJECTIVE: To investigate the expression variation of RAR-beta2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. METHODS: Nickel subsulfide (Ni(3)S(2)) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. RESULTS: The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. CONCLUSION: These findings suggest that loss of function or decrease of RAR-beta2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.


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CRRD Object Information
CRRD ID: 8552662
Created: 2014-04-23
Species: All species
Last Modified: 2014-04-23
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.