Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines.

Authors: Yamazaki, H  Kojima, N  Kato, K  Hirose, E  Iwasaki, T  Mizui, T  Takahashi, H  Hanamura, K  Roppongi, RT  Koibuchi, N  Sekino, Y  Mori, N  Shirao, T 
Citation: Yamazaki H, etal., J Neurochem. 2014 Feb;128(4):507-22. doi: 10.1111/jnc.12486. Epub 2013 Nov 13.
Pubmed: (View Article at PubMed) PMID:24117785
DOI: Full-text: DOI:10.1111/jnc.12486

Dendritic spines are small, actin-rich protrusions on dendrites, the development of which is fundamental for the formation of neural circuits. The actin cytoskeleton is central to dendritic spine morphogenesis. Drebrin is an actin-binding protein that is thought to initiate spine formation through a unique drebrin-actin complex at postsynaptic sites. However drebrin overexpression in neurons does not increase the final density of dendritic spines. In this study, we have identified and characterized a novel drebrin-binding protein, spikar. Spikar is localized in cell nuclei and dendritic spines, and accumulation of spikar in dendritic spines directly correlates with spine density. A reporter gene assay demonstrated that spikar acts as a transcriptional co-activator for nuclear receptors. We found that dendritic spine, but not nuclear, localization of spikar requires drebrin. RNA-interference knockdown and overexpression experiments demonstrated that extranuclear spikar regulates dendritic spine density by modulating de novo spine formation and retraction of existing spines. Unlike drebrin, spikar does not affect either the morphology or function of dendritic spines. These findings indicate that drebrin-mediated postsynaptic accumulation of spikar regulates spine density, but is not involved in regulation of spine morphology.

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CRRD ID: 8554267
Created: 2014-05-08
Species: All species
Last Modified: 2014-05-08
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.