Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.

Authors: Groffen, AJ  Martens, S  Diez Arazola, R  Cornelisse, LN  Lozovaya, N  De Jong, AP  Goriounova, NA  Habets, RL  Takai, Y  Borst, JG  Brose, N  McMahon, HT  Verhage, M 
Citation: Groffen AJ, etal., Science. 2010 Mar 26;327(5973):1614-8. doi: 10.1126/science.1183765. Epub 2010 Feb 11.
Pubmed: (View Article at PubMed) PMID:20150444
DOI: Full-text: DOI:10.1126/science.1183765

Synaptic vesicle fusion in brain synapses occurs in phases that are either tightly coupled to action potentials (synchronous), immediately following action potentials (asynchronous), or as stochastic events in the absence of action potentials (spontaneous). Synaptotagmin-1, -2, and -9 are vesicle-associated Ca2+ sensors for synchronous release. Here we found that double C2 domain (Doc2) proteins act as Ca2+ sensors to trigger spontaneous release. Although Doc2 proteins are cytosolic, they function analogously to synaptotagmin-1 but with a higher Ca2+ sensitivity. Doc2 proteins bound to N-ethylmaleimide-sensitive factor attachment receptor (SNARE) complexes in competition with synaptotagmin-1. Thus, different classes of multiple C2 domain-containing molecules trigger synchronous versus spontaneous fusion, which suggests a general mechanism for synaptic vesicle fusion triggered by the combined actions of SNAREs and multiple C2 domain-containing proteins.

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CRRD ID: 8554647
Created: 2014-05-08
Species: All species
Last Modified: 2014-05-08
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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.