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Effect of high glucose concentration on VEGF and PEDF expression in cultured retinal Muller cells.

Authors: Mu, H  Zhang, XM  Liu, JJ  Dong, L  Feng, ZL 
Citation: Mu H, etal., Mol Biol Rep. 2009 Nov;36(8):2147-51. doi: 10.1007/s11033-008-9428-8. Epub 2008 Dec 24.
Pubmed: (View Article at PubMed) PMID:19107574
DOI: Full-text: DOI:10.1007/s11033-008-9428-8

To explore the effect of high glucose concentration on the expression of vascular endothelial growth factor (VEGF) and pigment epithelium derived factor (PEDF) in the cultured rat retinal Muller cells. Rat Muller cells were cultured and RT-PCR and Western-blot analysis were used to measure the levels of VEGF and PEDF in cultured Muller cells at different high glucose concentrations. Under 10, 20, 30 mmol/L high glucose conditions, the levels of VEGF mRNA and protein increased and the levels of PEDF mRNA and protein decreased. These results suggest that the VEGF and PEDF expression in Muller cells are unbalance under high glucose concentration, which contribute to retinal neovascularization in diabetic retinopathy.

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CRRD Object Information
CRRD ID: 8655564
Created: 2014-05-15
Species: All species
Last Modified: 2014-05-15
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.