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Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding.

Authors: Wei, HM  Hu, HH  Chang, GY  Lee, YJ  Li, YC  Chang, HH  Li, C 
Citation: Wei HM, etal., FEBS Lett. 2014 May 2;588(9):1542-8. doi: 10.1016/j.febslet.2014.03.052. Epub 2014 Apr 12.
Pubmed: (View Article at PubMed) PMID:24726729
DOI: Full-text: DOI:10.1016/j.febslet.2014.03.052

Cellular nucleic acid binding protein (CNBP) contains seven zinc finger (ZF) repeats and an arginine and glycine (RG) rich sequence between the first and the second ZF. CNBP interacts with protein arginine methyltransferase PRMT1. Full-length but not RG-deleted or mutated CNBP can be methylated. Treatment with a methylation inhibitor AdOx reduced CNBP methylation, but did not affect the concentrated nuclear localization of CNBP. Nevertheless, arginine methylation of CNBP appeared to interfere with its RNA binding activity. Our findings show that arginine methylation of CNBP in the RG motif did not change the subcellular localization, but regulated its RNA binding activity.


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CRRD Object Information
CRRD ID: 8693365
Created: 2014-07-11
Species: All species
Last Modified: 2014-07-11
Status: ACTIVE


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