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Cloning and expression of a novel rat GABAA receptor.

Authors: Lolait, SJ  O'Carroll, AM  Kusano, K  Muller, JM  Brownstein, MJ  Mahan, LC 
Citation: Lolait SJ, etal., FEBS Lett. 1989 Mar 27;246(1-2):145-8.
Pubmed: (View Article at PubMed) PMID:2540033

Two full-length cDNA clones encoding alpha- and beta-subunits of a GABAA receptor have been isolated from a rat cerebral cortex cDNA library. The mature alpha-subunit protein consists of 428 amino acids with a calculated Mr of 48,680. This protein is highly homologous (approximately 99% amino acid identity) with the bovine brain alpha 1-subunit receptor [(1988) Nature 335, 76-79]. The mature rat beta-subunit receptor is a 448 amino acid polypeptide and shares approximately 80% amino acid identity with the previously characterized bovine GABAA receptor beta-subunit [(1987) Nature 328, 221-227]. Co-expression of the cloned DNA in Xenopus oocytes produces a functional receptor and ion channel with pharmacological characteristics of a GABAA receptor. GABAA alpha- and beta-subunit mRNA is detectable in the cortex, cerebellum and hippocampus.


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CRRD Object Information
CRRD ID: 8693372
Created: 2014-07-11
Species: All species
Last Modified: 2014-07-11
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.