RGD Reference Report - On how mammalian transcription factors recognize methylated DNA. - Chinchilla Research Resource Database
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On how mammalian transcription factors recognize methylated DNA.

Authors: Buck-Koehntop, BA  Defossez, PA 
Citation: Buck-Koehntop BA and Defossez PA, Epigenetics. 2013 Feb;8(2):131-7. doi: 10.4161/epi.23632. Epub 2013 Jan 16.
CRRD ID: 8695954
Pubmed: (View Article at PubMed) PMID:23324617
DOI: Full-text: DOI:10.4161/epi.23632

DNA methylation is an epigenetic mark that is essential for the development of mammals; it is frequently altered in diseases ranging from cancer to psychiatric disorders. The presence of DNA methylation attracts specialized methyl-DNA binding factors that can then recruit chromatin modifiers. These methyl-CpG binding proteins (MBPs) have key biological roles and can be classified into three structural families: methyl-CpG binding domain (MBD), zinc finger, and SET and RING finger-associated (SRA) domain. The structures of MBD and SRA proteins bound to methylated DNA have been previously determined and shown to exhibit two very different modes of methylated DNA recognition. The last piece of the puzzle has been recently revealed by the structural resolution of two different zinc finger proteins, Kaiso and ZFP57, in complex with methylated DNA. These structures show that the two methyl-CpG binding zinc finger proteins adopt differential methyl-CpG binding modes. Nonetheless, there are similarities with the MBD proteins suggesting some commonalities in methyl-CpG recognition across the various MBP domains. These fresh insights have consequences for the analysis of the many other zinc finger proteins present in the genome, and for the biology of methyl-CpG binding zinc finger proteins.


Molecular Pathway Annotations    
Objects Annotated

Genes (Homo sapiens)
MBD1  (methyl-CpG binding domain protein 1)
MBD2  (methyl-CpG binding domain protein 2)
MBD4  (methyl-CpG binding domain 4, DNA glycosylase)
MECP2  (methyl-CpG binding protein 2)
UHRF1  (ubiquitin like with PHD and ring finger domains 1)
UHRF2  (ubiquitin like with PHD and ring finger domains 2)
ZBTB33  (zinc finger and BTB domain containing 33)
ZBTB38  (zinc finger and BTB domain containing 38)
ZBTB4  (zinc finger and BTB domain containing 4)
ZFP57  (ZFP57 zinc finger protein)

Additional Information



RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.