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Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells.

Authors: Tzeng, SL  Cheng, YW  Li, CH  Lin, YS  Hsu, HC  Kang, JJ 
Citation: Tzeng SL, etal., J Biol Chem. 2006 Jun 2;281(22):15405-11. Epub 2006 Mar 28.
Pubmed: (View Article at PubMed) PMID:16569639
DOI: Full-text: DOI:10.1074/jbc.M601807200

Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co-immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.

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CRRD Object Information
CRRD ID: 9587838
Created: 2014-10-21
Species: All species
Last Modified: 2014-10-21
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.