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DNA demethylation regulates the expression of miR-210 in neural progenitor cells subjected to hypoxia.

Authors: Xiong, L  Wang, F  Huang, X  Liu, ZH  Zhao, T  Wu, LY  Wu, K  Ding, X  Liu, S  Wu, Y  Zhao, Y  Zhu, LL  Fan, M 
Citation: Xiong L, etal., FEBS J. 2012 Dec;279(23):4318-26. doi: 10.1111/febs.12021. Epub 2012 Nov 7.
Pubmed: (View Article at PubMed) PMID:23039253
DOI: Full-text: DOI:10.1111/febs.12021

Several studies have identified a set of hypoxia-regulated microRNAs, among which is miR-210, whose expression is highly induced by hypoxia in various cancer cell lines. Recent studies have highlighted the importance of miR-210 and its transcriptional regulation by the transcription factor hypoxia-inducible factor-1 (HIF-1). We report here that the expression of miR-210 was highly induced in neural progenitor cells (NPCs) subjected to hypoxia. Specifically, treating hypoxic NPCs with the DNA demethylating agent 5-aza-2'-deoxycytidine significantly increased the expression of miR-210, even under normoxia; however, the activity of hypoxia-inducible factor-1 was unaffected. Further analysis of the miR-210 sequence revealed that it is embedded in a CpG island. Bisulfite sequencing of the miR-210 CpG island from NPCs grown under hypoxic conditions showed 24% CpG methylation in NPCs exposed to 20% O(2) , 18% in NPCs exposed to 3% O(2) , and 12% in NPCs exposed to 0.3% O(2) . In addition, the activity of DNA methyltransferases (DNMTs) in NPCs decreased after exposure to hypoxia. Specifically, the expression of DNMT3b decreased significantly after exposure to 0.3% O(2) . Thus, these results demonstrate that DNA demethylation regulates miR-210 expression in NPCs under both normoxia and hypoxia.


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CRRD Object Information
CRRD ID: 9589076
Created: 2014-11-06
Species: All species
Last Modified: 2014-11-06
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.