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Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain.

Authors: Malherbe, P  Alberati-Giani, D  Kohler, C  Cesura, AM 
Citation: Malherbe P, etal., FEBS Lett. 1995 Jun 26;367(2):141-4.
Pubmed: (View Article at PubMed) PMID:7796908

A soluble aminotransferase with kynurenine aminotransferase (KAT) activity has been recently isolated from rat brain. This enzyme corresponds to a cytosolic form of glutamine transaminase K (GTK). In addition to the cytosolic enzyme, a mitochondrial-associated form of this KAT/GTK also exists. In the present work we have isolated a rat brain cDNA clone encoding a KAT/GTK enzyme identical to the soluble form but carrying an additional stretch of 32 amino acids at its NH2-terminus. Several structural features of this sequence resemble those of leader peptides for mitochondrial import. Evidence that the isolated cDNA encoded for mitochondrial KAT/GTK was obtained after transfection of HEK-293 cells with the cDNA coding for this new KAT/GTK isoenzyme. In fact, a significant enrichment of both KAT and GTK enzymatic activities was found in the crude mitochondrial fraction of the transfected cells.


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CRRD Object Information
CRRD ID: 9685040
Created: 2014-12-15
Species: All species
Last Modified: 2014-12-15
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.