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The expression of angiotensin-converting enzyme 2-angiotensin-(1-7)-Mas receptor axis are upregulated after acute cerebral ischemic stroke in rats.

Authors: Lu, J  Jiang, T  Wu, L  Gao, L  Wang, Y  Zhou, F  Zhang, S  Zhang, Y 
Citation: Lu J, etal., Neuropeptides. 2013 Oct;47(5):289-95. doi: 10.1016/j.npep.2013.09.002. Epub 2013 Sep 18.
Pubmed: (View Article at PubMed) PMID:24090950
DOI: Full-text: DOI:10.1016/j.npep.2013.09.002

There is now unequivocal evidence that the angiotensin-converting enzyme 2(ACE2)-Ang-(1-7)-Mas axis is a key component of the renin-angiotensin system (RAS) cascade, which is closely correlated with ischemic insult occurrence. Our previous studies demonstrated that the Ang-(1-7), was an active member of the brain RAS. However, the ACE2-Ang-(1-7)-Mas axis expression after cerebral ischemic injury are currently unclear. In the present study, we investigated the time course of ACE2-Ang-(1-7) and Mas receptor expression in the acute stage of cerebral ischemic stroke. The content of Ang-(1-7) in ischemic tissues and blood serum was measured by specific EIA kits. Real-time PCR and western blot were used to determine messenger RNA (mRNA) and protein levels of the ACE2 and Mas. The cerebral ischemic lesion resulted in a significant increase of regional cerebral and circulating Ang-(1-7) at 6-48 h compared with sham operation group following focal ischemic stroke (12h: 7.276+/-0.320 ng/ml vs. 2.466+/-0.410 ng/ml, serum; 1.024+/-0.056 ng/mg vs. 0.499+/-0.032, brain) (P<0.05). Both ACE2 and Mas expression were markedly enhanced compared to the control in the ischemic tissues (P<0.05). Mas immunopositive neurons were also seen stronger expression in the ischemic cortex (19.167+/-2.858 vs. 7.833+/-2.483) (P<0.05). The evidence collected in our present study will indicate that, ACE2-Ang-(1-7)-Mas axis are upregulated after acute ischemic stroke and would play a pivotal role in the regulation of acute neuron injury in ischemic cerebrovascular diseases.

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CRRD Object Information
CRRD ID: 9685436
Created: 2015-01-08
Species: All species
Last Modified: 2015-01-08
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.