Recombinant expression of perchloric acid-soluble protein reduces cell proliferation.

Authors: Kanouchi, H  Tachibana, H  Oka, T  Yamada, K 
Citation: Kanouchi H, etal., Cell Mol Life Sci. 2001 Aug;58(9):1340-3.
Pubmed: (View Article at PubMed) PMID:11577990
DOI: Full-text: DOI:10.1007/PL00000945

Perchloric acid-soluble protein (PSP) may play an important role in the regulation of cellular physiological functions because it has been highly conserved throughout evolution; however, this role has not been well elucidated. In previous reports, we suggested that PSP regulates cell proliferation. In this study, we examined the effect of PSP expression on proliferation of the normal rat kidney cell line NRK-52E, the rat hepatocyte cell line RLN-10, and the rat hepatoma cell line dRLh-84. Cells transfected with pcDNA-sense-PSP (pcDNA-S-PSP) over-expressed PSP mRNA and protein, and cell proliferation of the transfected cells was suppressed compared with that of cells transfected with pcDNA-empty (pcDNA-E). Cell viability of pcDNA-S-PSP-transfected cells was similar to that of pcDNA-E-transfected cells. Thus, over-expression of PSP suppresses cell proliferation without any influence on cell viability. These findings are the first to report an inhibitory activity of PSP on cell proliferation.


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CRRD ID: 9685551
Created: 2015-01-19
Species: All species
Last Modified: 2015-01-19
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.