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[Expression of nucleolin in pressure overload-induced cardiac hypertrophy rats].

Authors: Yan, S  Wu, S  Sun, L  Jiang, B  Tu, Z  Xiao, X 
Citation: Yan S, etal., Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 Feb;39(2):124-8. doi: 10.11817/j.issn.1672-7347.2014.02.003.
Pubmed: (View Article at PubMed) PMID:24608397
DOI: Full-text: DOI:10.11817/j.issn.1672-7347.2014.02.003

OBJECTIVE: To detect the expression of nucleolin in cardiac hypertrophy rats induced by pressure overload. METHODS: A total of 40 SD rats with body weight 180 g and 220 g were recruited and randomly divided into 2 groups: a transverse aortic constriction (TAC) group and a sham surgery group. Cardiac hypertrophy model was employed by transverse aortic constriction surgery. Then 2 weeks and 4 weeks after the experiment, the heart mass index (HMI), left ventricle mass index (LVMI) were measured. beta-MHC mRNA in the heart tissue was detected with RT-PCR. Nucleolin in the heart, brain and kidney was respectively detected with Western blot. RESULTS: Compared with the sham surgery group, HMI, LVMI in the TAC group increased significantly (P<0.01) 4 weeks after the surgery; the expression of beta-MHC mRNA in the heart tissue increased (P<0.05) in the TAC group 4 weeks after the surgery; and the expression of nucleolin protein in the heart tissue of the TAC group was remarkably upregulated (P<0.05) 2 weeks after the surgery, with no change in the brain and kidney tissue between the 2 groups. CONCLUSION: Expression of nucleolin protein has been upregulated in response to pressure overload, which may suggest that nucleolin plays a role in cardiac hypertrophy induced by pressure overload.


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CRRD Object Information
CRRD ID: 9686390
Created: 2015-02-05
Species: All species
Last Modified: 2015-02-05
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.