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Kinetics of neurotensin gene expression in the developing gut.

Authors: Evers, BM  Wang, XF 
Citation: Evers BM and Wang XF, Surgery. 1996 Feb;119(2):186-90.
Pubmed: (View Article at PubMed) PMID:8571204

BACKGROUND: Expression of the gene encoding neurotensin (NT/N) is regulated in a strict temporal- and spatial-specific pattern during gut development; the mechanisms (that is, transcriptional versus posttranscriptional) responsible for this expression pattern are not known. The purpose of this study was to determine whether developmental changes in NT/N expression reflect alternations in gene transcription. METHODS: Sensitive ribonuclease protection assays were performed with a rat NT/N genomic probe containing the entire sequence of both exon 1 and intron 1 hybridized with RNA from fetal (day 19) and postnatal (days 14, 28, and 60) rat jejunum and ileum; signals were quantitated densitometrically. RESULTS: Mature (exon 1) and precursor (exon 1 + intron 1) NT/N RNA, initially low in the fetus, increased dramatically by postnatal day 14 and attained maximal levels by day 28. NT/N RNA levels remained stable in the ileum of the 60-day-old rat but decreased in the jejunum, consistent with the typical expression pattern in the gut. CONCLUSIONS: Concomitant changes in expression of precursor and mature NT/N RNA suggest that NT/N gene regulation occurs at the level of transcription in the gut during development. Identifying the factors that regulate NT/N gene transcription is crucial to our understanding of how neurotensin functions in the gut.


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CRRD Object Information
CRRD ID: 9743850
Created: 2015-02-18
Species: All species
Last Modified: 2015-02-18
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.