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Expression of the high-affinity choline transporter CHT1 in epithelia.

Authors: Pfeil, U  Haberberger, RV  Lips, KS  Eberling, L  Grau, V  Kummer, W 
Citation: Pfeil U, etal., Life Sci. 2003 Mar 28;72(18-19):2087-90.
Pubmed: (View Article at PubMed) PMID:12628461

Uptake of choline by the high-affinity choline transporter CHT1 is the rate-limiting step in neuronal acetylcholine (ACh) synthesis. Here, we investigated by RT-PCR, in-situ hybridisation, immunohistochemistry, and Western blotting whether CHT1 is also expressed in cholinergic epithelia. CHT1-mRNA and -protein were detected in keratinocytes of human skin, rat skin and tongue, the human keratinocyte cell line HaCaT, and the ciliated cells of the rat tracheal epithelium. Immunohistochemically, CHT1 was predominantly localized to the epithelial cell membranes, in case of ciliated tracheal cells it was restricted to the apical membrane. This is the first study to demonstrate the expression of CHT1 in non-neuronal cells. The close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia.


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CRRD Object Information
CRRD ID: 9999384
Created: 2015-04-16
Species: All species
Last Modified: 2015-04-16
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.