Lard-based high-fat diet increases secretory leukocyte protease inhibitor expression and attenuates the inflammatory response of acute lung injury in endotoxemic rats.

Authors: Fujiwara, M  Miyoshi, M  Sakai, S  Nishiokada, A  Aoyama-Ishikawa, M  Maeshige, N  Usami, Y  Hamada, Y  Takahashi, M  Usami, M 
Citation: Fujiwara M, etal., Clin Nutr. 2014 Nov 8. pii: S0261-5614(14)00263-5. doi: 10.1016/j.clnu.2014.11.001.
Pubmed: (View Article at PubMed) PMID:25466948
DOI: Full-text: DOI:10.1016/j.clnu.2014.11.001

BACKGROUND & AIMS: Acute lung injury (ALI) is less severe in obese than in nonobese patients, but the mechanism is unclear. Secretory leukocyte protease inhibitor (SLPI) is the key anti-inflammatory protein in various lung diseases. We have previously reported changes of the surgical stress in obese rats using lard-based high-fat diet (HFD). The purpose of this study was to elucidate the effect of lard-based HFD on the pathophysiology of lipopolysaccharide (LPS)-induced ALI, and the role of SLPI expression. METHODS: Male Wistar rats were fed lard-based HFD (60 kcal% fat) or control diet (CD) for either 4 or 12 weeks and were killed after intraperitoneal LPS injection. Analyses included messenger RNA expression of TNF-alpha, macrophage inflammatory protein (MIP)-2, inducible nitric oxide synthase (iNOS), IL-10 and SLPI in the lung tissue and bronchoalveolar lavage fluid, and histology of the lungs. RESULTS: Rats fed HFD for 12 weeks showed suppression of the lung injury and oxidative stress after LPS injection, as indicated by reduction of pulmonary TNF-alpha, MIP-2 and iNOS mRNA expression and 8-hydroxy-2'-deoxyguanosine immunostaining. The increased pulmonary SLPI caused by lard was associated with decreased pro-inflammatory cytokines and oxidative stress, which eventually resulted in the prevention of ALI. Those effects of lard on LPS-induced ALI were greater after 12 weeks than after 4 weeks feeding, as indicated by the reduction of TNF-alpha, MIP-2 and iNOS levels. CONCLUSIONS: Feeding lard-based HFD for 12 weeks attenuated LPS-induced ALI with increased pulmonary SLPI expression in rats.

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CRRD Object Information
CRRD ID: 9999425
Created: 2015-04-17
Species: All species
Last Modified: 2015-04-17
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.